The lab says fixation will trigger the dyes to leak from your cells. I do think the idea is fixation might harm mobile membranes. I did discover a paper which describes fixation using methanol or acetic acid, after staining by using a DCFDA derivative. DCFDA is definitely the reagent that https://collinbjnsx.blogcudinti.com/27935900/manual-article-review-is-required-for-this-article